O lenei pusa o loʻo faʻaaogaina mo in vitro qualitative nucleic acid detection ofMonkeypox Virus i le sua o le tagata, fa'ata'ita'iga o le fa'ama'i mafa'ata'ita'iga pa'u.O seti muamua ma fa'ailoga fa'ailoga FAM ua mamanuinamo su'esu'ega fa'apitoa ole Monkeypox Virus, Human RNase P geneaumai fa'atasi ma le fa'ata'ita'iga fa'ata'ita'iga e maua ai se totonupulea e faʻamaonia le faʻaogaina o le nucleic extraction procedure ma reagentamiosa'o.O su'esu'ega e fa'atatau i le kenera RNase P tagata ua fa'ailogaina i le VIC.
| Vaega | 48 Su'ega/pusa | 96 Su'ega/pusa |
| PCR tali fa'afefe | 672 μL × 1 paipa | 672 μL × 2 paipa |
| PCR enzyme fefiloi | 50 μL × 1 paipa | 100 μL × 1 paipa |
| Pulea lelei | 100 μL × 1 paipa | 200 μL × 1 paipa |
| Le pulea lelei | 100 μL × 1 paipa | 200 μL × 1 paipa |
1. Ma'ale'ale: 200 kopi/mL.
2. Fa'apitoa: Leai se tali fa'afeagai ma Enterovirus(EV), Miselavirus(MV), Rubella virus(RV), Varicella-zoster virus(VZV),Dengue virus(DenV), TagataParvovirus B19(HPVB19),Epstein-barr virus (EBv), siama herpes tagata 6(HHV-6)
3. Sa'o: CV ≤ 5%.
Taimi Moni PCR System: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad/CFX96 Touch ，Hongshi SLAN-96S/96P，AGS8830, AGS4800